Anti-PR Antibody

The progesterone receptor (PR) is a nuclear hormone receptor that mediates progesterone-driven transcriptional programs in reproductive tissues, the mammary gland, and hormone-responsive tumors. The polyclonal anti-PR reagent from Antibodies.com is an affinity tool for detecting this receptor in fixed tissue sections and prepared cell lysates.

Validated applications include Western blot and immunohistochemistry (WB, IHC), with reactivity confirmed against human, mouse, and rat samples. Each vial contains 100 µl of antibody at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide, at approximately pH 7.2, ready to dilute into standard blocking or antibody diluents for downstream assays.

Raised in rabbit as a polyclonal, the reagent recognizes multiple epitopes across the PR protein, an approach that can improve detection of low-abundance targets and tolerate minor sequence variation between isoforms such as PRA and PRB. The unconjugated format leaves the choice of fluorophore, enzyme, or biotin label to the user, supporting both chromogenic IHC staining and chemiluminescent Western blot detection on nitrocellulose or PVDF membranes.

For in vitro research use, the antibody supports studies of hormone-dependent cancers including breast and endometrial carcinoma, reproductive tissue profiling, and signaling pathway analysis in progesterone-responsive cell lines. Storage: refrigerate at 4°C for short-term use, or freeze aliquots at -20°C for longer-term storage; limit freeze-thaw cycles to preserve activity, and protect from light where applicable. The vial format also supports single-use aliquoting so working stocks can be extended across multiple experiments without compromising the parent vial.