Anti-PPP4R4 Antibody

The polyclonal rabbit IgG reagent offered by Antibodies.com targets PPP4R4, a regulatory subunit of serine/threonine-protein phosphatase 4, and is validated for western blot detection in mouse-derived samples. PPP4R4, also referenced in the literature as SMEK2, directs substrate specificity and subcellular localization of the PP4 catalytic complex, contributing to pathways that include DNA damage checkpoint control, centrosome maturation, and neuronal signaling. Laboratories investigating PP4-dependent dephosphorylation cascades in murine models can apply this unconjugated primary antibody at working dilutions of 1:500 to 1:2000 to visualize the endogenous protein from cultured cell lysates or fresh tissue homogenates. The antibody is supplied as 50 µl of liquid in a single vial, an amount sufficient for roughly 10 to 20 western blot experiments depending on membrane dimensions, transfer efficiency, and blocking conditions. Store the vial at -20 °C in small aliquots to limit repeated freeze-thaw cycles, and keep the reagent protected from light when it is not actively in use. The polyclonal IgG formulation provides epitope coverage spanning multiple regions of the target protein, supporting reliable signal development with common HRP-conjugated or fluorescent secondary antibodies. This reagent is intended for research use only and is not cleared for diagnostic or therapeutic applications.