Anti-PPP4C Antibody

PPP4C (Protein Phosphatase 4 Catalytic Subunit) is a serine/threonine phosphatase implicated in cell cycle progression, DNA damage response signaling, microtubule organization at the centrosome, and spliceosomal assembly. This unconjugated primary antibody from Antibodies.com was raised in rabbit and recognizes the endogenous PPP4C protein in human, mouse, and rat samples, supporting cross-species experimental design in phosphatase research.

The reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, delivered in a 100 µl vial sized for multiple immunoblotting and imaging experiments. Validated applications include western blot, immunohistochemistry, and immunofluorescence, making the reagent a flexible tool for studies of phosphatase abundance and subcellular localization in dividing cells and stressed tissue.

For routine handling, keep the vial refrigerated; the 50% glycerol component helps shield the antibody from freeze-thaw damage during repeated aliquoting. Researchers commonly employ this reagent to probe PPP4C levels following siRNA knockdown, CRISPR-based gene editing, or chemical inhibition of upstream kinases, and to track relocalization of the phosphatase during mitosis and the DNA damage response.