Anti-PPP2R5A Antibody

Produced in rabbit and affinity-purified, this polyclonal antibody from Antibodies.com recognizes PPP2R5A, the B-alpha regulatory subunit of serine/threonine protein phosphatase 2A (PP2A). PP2A is a major cellular phosphatase complex that controls cell cycle progression, apoptosis, and MAPK signaling, with the B-family subunits directing substrate selectivity and subcellular targeting of the heterotrimeric holoenzyme.

Each vial contains 100 µl of unconjugated antibody at 1 mg/ml in phosphate buffered saline (PBS) supplemented with 0.05% sodium azide at approximately pH 7.2. The reagent has been validated for Western blot and immunocytochemistry, with reactivity confirmed against human, mouse, and rat samples, supporting cross-species comparative work in cell biology and signal transduction research.

Typical use cases include probing PP2A complex assembly in cultured mammalian cells, mapping B-subunit distribution across tissue panels, and characterizing phosphatase regulation downstream of growth factor and stress pathways. For best results, aliquot upon first thaw and refrigerate between uses to preserve antibody activity across multiple experiments.