Anti-PPP2R1B Antibody

Encoded by a gene frequently mutated in lung and colorectal carcinomas, PPP2R1B contributes the beta isoform of the PP2A scaffold A subunit and has been characterized in the literature as a candidate tumor suppressor. This unconjugated polyclonal antibody from Antibodies.com, raised in rabbit, enables detection of human PPP2R1B in Western blot and immunohistochemistry workflows. The reagent is supplied as 100 µl of liquid, supporting studies of PP2A holoenzyme assembly, phosphatase signaling, and isoform-specific substrate recognition in cancer cell lines and formaldehyde-fixed tissue sections.

The polyclonal format allows recognition of multiple epitopes across PPP2R1B, which strengthens signal in denatured lysate-based assays. For Western blot, loading 20–50 µg of total protein and probing with an HRP-conjugated secondary antibody yields chemiluminescent detection; for IHC on paraffin sections, citrate-buffer antigen retrieval at pH 6 combined with an HRP-polymer system generally produces specific nuclear and cytoplasmic staining.

Common experimental applications include scoring PPP2R1B expression in tumor tissue microarrays, comparing A-subunit isoform abundance across patient-derived samples, and probing how loss of PP2A scaffolding alters downstream dephosphorylation of substrates in the Wnt and MAPK pathways. Store the vial refrigerated between uses and avoid repeated freeze-thaw cycles to preserve antibody activity.