Anti-PPP2R1A Antibody

Raised in rabbit and purified by affinity chromatography against the immunogen, this polyclonal antibody from Antibodies.com targets the PPP2R1A subunit of protein phosphatase 2A (PP2A), also known as the 61 kDa A isoform PR65-alpha or PP2A subunit A isoform R1-alpha. The reagent is supplied at 1.0 mg/mL in a 50 µL volume, formulated in phosphate-buffered saline at pH 7.4 (without Mg²⁺ and Ca²⁺) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. It is unconjugated and detects endogenous levels of total PPP2R1A protein in samples derived from human, mouse, and rat sources. Recommended applications include Western blotting (WB) and immunocytochemistry/immunofluorescence (ICC/IF), making it suitable for studies of PP2A complex composition and regulation across a range of cell- and tissue-based assays. The immunogen used for immunization is a recombinant protein of human PPP2R1A, supporting robust recognition of the native target. For handling, the vial is shipped at 4 °C; upon receipt it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Intended for research use only, this affinity-purified polyclonal reagent provides a reliable tool for researchers investigating PP2A scaffolding subunit biology in signalling, cell-cycle, and chromatin-related pathways.