Anti-PPP2CA Antibody

The PPP2CA gene encodes the catalytic alpha subunit of protein phosphatase 2A (PP2A), one of the most abundant serine/threonine phosphatases in eukaryotic cells. PP2A regulates a broad network of cellular signaling cascades governing cell cycle progression, apoptosis, and mitogenic growth factor responses, and its dysfunction has been implicated in neurodegeneration and oncogenesis. This rabbit polyclonal antibody from Antibodies.com is raised against PPP2CA and is validated for Western blot and immunohistochemistry workflows on human and mouse samples.

Supplied as 100 µl of unconjugated antiserum, the reagent can be directly conjugated by the researcher to HRP, fluorophore, or biotin tags of their choosing, supporting flexible detection strategies across immunoblotting and tissue staining protocols. Polyclonal reactivity against multiple epitopes of the PPP2CA catalytic subunit increases the likelihood of recognizing the target even when post-translational modifications or partial proteolysis are present, which is useful for studying phosphorylation dynamics and tumor suppressor pathways.

For optimal antibody stability, the vial contents should be aliquoted upon arrival and stored at -20 °C, with limited freeze-thaw cycles to preserve binding activity over long-term use. Researchers investigating PP2A holoenzyme assembly, regulatory B subunit interactions, or disease-associated phosphatase activity will find this reagent a reliable tool for basic and translational research.