Anti-PPP1R1B (phospho T75) Antibody

The target protein PPP1R1B, widely known by its alias DARPP-32 (dopamine- and cAMP-regulated neuronal phosphoprotein, 32 kDa), sits at a central node of dopaminergic signal transduction in striatal medium spiny neurons. This unconjugated rabbit polyclonal IgG from Antibodies.com was raised against the phosphorylated threonine 75 epitope, a residue modified by cyclin-dependent kinase 5 (CDK5) that gates downstream AMPA and NMDA receptor trafficking. Validated for immunofluorescence (IF), the reagent binds human, mouse, and rat samples and is supplied as a 50 µl aliquot for labeling perfusion-fixed brain sections, cultured striatal neurons, or freshly dissociated tissue preparations. The polyclonal format tolerates minor local variation around the phosphoepitope, which is useful when working with methanol- or paraformaldehyde-fixed specimens from Parkinson's disease models, schizophrenia postmortem samples, and chronic drug-exposure paradigms. Investigators use this antibody to chart CDK5-driven phosphorylation dynamics during corticostriatal plasticity, reward learning, and dopaminergic degeneration, often alongside western blot or immunohistochemistry workflows that probe total DARPP-32 abundance.