Anti-PPP1CB Antibody

Manufactured by Antibodies.com, this affinity-purified rabbit polyclonal reagent is raised against protein phosphatase 1 catalytic subunit beta (PPP1CB), a ubiquitously expressed serine/threonine phosphatase implicated in cell-cycle progression, glycogen metabolism, and neuronal signalling. The antibody is supplied as a 50 µl aliquot at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, a buffer system that permits convenient storage at –20 °C and minimises freeze–thaw losses. The host species is rabbit, the clonality is polyclonal, the immunoglobulin isotype is IgG, and the preparation is unconjugated, leaving the reporter choice open to the end user for chromogenic, fluorescent, or enzymatic detection schemes. Validated applications on the supplier's product page include Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), making the reagent suitable for both denaturing lysate-based assays and fixed-tissue or fixed-cell imaging workflows. Cross-reactivity has been confirmed in Human, Mouse, and Rat samples, so a single lot can support comparative work across the most common mammalian model systems. The antibody recognises endogenous levels of total PPP1CB protein following affinity purification against the immunogen, supporting robust signal-to-noise in routine loading controls and target-modulation studies. Each vial ships at a concentration that allows direct dilution into standard blocking buffers; for example, typical WB working dilutions of 1:500–1:2,000 and IHC/IF dilutions of 1:50–1:200 are achievable from a single 50 µl stock. Researchers should avoid prolonged exposure to light because of the azide-preserved buffer, and brief centrifugation prior to opening is recommended to recover the full volume from the cap.