Anti-PPP1CA Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent targets the serine/threonine-protein phosphatase PP1-alpha catalytic subunit encoded by PPP1CA (also referenced as PP-1A or PPP1A). Supplied at 1 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, the unconjugated antibody is offered in a 50 µL pack size and is intended for research use only. It was raised against a recombinant human PPP1CA immunogen and purified by affinity chromatography, ensuring recognition of the endogenous total PPP1CA protein. Validated applications include Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), with cross-species reactivity confirmed in human, mouse, and rat samples. Published example data illustrate Western blot detection across multiple cell lines, IHC staining of paraffin-embedded human kidney and stomach cancer tissue at a 1:200 dilution, and immunofluorescence imaging of U2OS cells with DAPI nuclear counterstain. The reagent should be stored at –20 °C to preserve stability, and laboratory workflows benefit from the unconjugated format, which permits flexible pairing with secondary detection chemistries of the researcher's choice.