Anti-PPOX Antibody

This polyclonal rabbit antibody targets protoporphyrinogen oxidase (PPOX), the seventh enzyme of the heme biosynthetic pathway. PPOX catalyzes the six-electron oxidation of protoporphyrinogen IX to protoporphyrin IX at the inner mitochondrial membrane, the penultimate step in producing the protoporphyrin IX precursor of heme. Loss-of-function variants in the PPOX gene cause variegate porphyria, a hereditary disorder of heme metabolism, which makes the protein a frequent target in porphyria diagnostics, mitochondrial enzyme biology, and studies of oxidative chemistry inside the organelle.

Provided as an unconjugated antibody preparation in a 100 µl vial, the reagent is raised in rabbit and validated for Western blot and immunofluorescence workflows. It is confirmed to react with human, mouse, and rat samples, supporting studies in established cell lines, primary cultures, and tissue sections across these three species. The unconjugated format leaves detection chemistry open, so the antibody can be paired with HRP, fluorophore, or biotin secondary reagents chosen by the researcher.

Typical experimental use includes confirming PPOX expression levels in cell lysates by Western blot, localizing the protein to mitochondria by immunofluorescence staining, and probing disease-associated variants in patient-derived material. The antibody should be stored refrigerated in working aliquots to preserve activity across repeated freeze-thaw cycles. Investigators working on heme biosynthesis disorders, mitochondrial dysfunction, or porphyria pathogenesis will find this Antibodies.com reagent a well-characterized tool for both qualitative and semi-quantitative analysis.