Anti-PPM1K Antibody

Raised in rabbit as an unconjugated polyclonal reagent, this primary antibody recognizes PPM1K (also designated PP2Cm), a mitochondrial serine/threonine phosphatase that dephosphorylates and thereby activates the branched-chain α-ketoacid dehydrogenase complex and gates branched-chain amino acid catabolism. Antibodies.com supplies the reagent at 100 µl per vial, validated in western blot and immunohistochemistry on human samples, with the immunogen region mapping within the PPM1K coding sequence. Endogenous PPM1K has been most often studied in liver, heart, and skeletal muscle tissue, where it couples BCAA flux to metabolic and cardiovascular phenotypes; laboratories using this reagent typically probe for an approximately 40 kDa band by WB or detect mitochondrial staining patterns by IHC. The format suits investigators mapping BCKD pathway regulation, branched-chain ketoacid dehydrogenase activity, and cellular models of maple syrup urine disease, cardiac hypertrophy, or insulin resistance, where altered PPM1K expression has been reported.