Anti-PPIF Antibody

Produced by Antibodies.com as a rabbit polyclonal reagent, this primary antibody targets peptidylprolyl isomerase F (PPIF), the mitochondrial matrix protein commonly referred to as Cyclophilin D. PPIF sits at the core of the mitochondrial permeability transition pore and is widely investigated in studies of calcium-dependent mitochondrial swelling, necrotic cell death, and the cellular response to oxidative stress.

This unconjugated antibody is supplied as 100 µL of liquid stock in a single vial and is validated for Western blot and immunohistochemistry workflows. Because the immunogen corresponds to a conserved region of PPIF, the antibody cross-reacts across three commonly used model systems, detecting endogenous protein in Human, Mouse, and Rat samples.

Recommended storage is at -20 °C in working aliquots to limit freeze-thaw cycles, with short-term use refrigerated at 4 °C for active stocks. The polyclonal format offers epitope diversity that can be useful when probing truncated or post-translationally modified forms of Cyclophilin D, and the unconjugated format leaves the choice of HRP, fluorophore, or biotin label open to the researcher.

Typical applications include verifying PPIF expression in cardiac ischemia-reperfusion models, characterizing mitochondrial fractions from neural tissue, and mapping Cyclophilin D localization by IHC in fixed sections from rodent and human tissue. Researchers studying mPTP opening, mitochondrial calcium handling, and programmed necrosis pathways will find this reagent a practical primary antibody for both discovery and validation experiments.