Anti-PPIE Antibody

The PPIE gene encodes Peptidyl-Prolyl Isomerase E, also known as cyclophilin E. As a member of the cyclophilin immunophilin family, the enzyme catalyzes cis-trans isomerization of peptide bonds preceding proline residues and contributes to U4/U6.U5 tri-snRNP assembly during pre-mRNA splicing. The protein also binds the immunosuppressant cyclosporin A and has been investigated in the context of spliceosome function and certain cancer cell lines.

This Antibodies.com reagent is a rabbit polyclonal IgG raised against PPIE, supplied unconjugated in a 100 µL vial. Validated applications cover Western blot and immunoprecipitation, and the antibody detects endogenous cyclophilin E in human, mouse, and rat samples.

• Western blot detection of PPIE expression across cell lysates and tissue homogenates
• Immunoprecipitation of native cyclophilin E for spliceosome characterization or interactome studies
• Investigation of pre-mRNA splicing regulation and cyclosporin-binding immunophilin signaling

Aliquot upon receipt and store at -20 °C; protect from repeated freeze-thaw cycles. For research use only.