Anti-PPIA Antibody

Primary antibody reagent targeting PPIA, the 18 kDa peptidyl-prolyl cis-trans isomerase A commonly referred to as Cyclophilin A. This unconjugated rabbit polyclonal antibody from Antibodies.com is validated for Western blot and immunohistochemistry workflows on samples derived from human, mouse, and rat origin. The reagent is supplied at 100 µl volume, supporting multiple runs in standard mini-gel and slide-based protocols. PPIA catalyzes the cis-trans isomerization of proline peptide bonds, facilitating nascent chain folding in the cytosol, and acts as the intracellular receptor for the immunosuppressant cyclosporine A. Detection of the endogenous protein underpins studies of T-cell signaling, viral entry mechanisms, and cytoskeletal remodeling during cancer cell migration. The polyclonal format recognizes multiple epitopes on the target, useful when probing truncated or post-translationally modified PPIA species. Recommended storage at -20 °C in working aliquots preserves activity across repeated freeze-thaw cycles; immunoblotting workflows typically load 20 to 40 µg of total lysate per lane to visualize the monomer near the 18 kDa marker.