Anti-PPARA Antibody

Raised in rabbits and purified as an unconjugated IgG, the Anti-PPARA Antibody distributed by Antibodies.com is a polyclonal reagent for researchers investigating peroxisome proliferator-activated receptor alpha, a ligand-activated nuclear receptor that governs fatty acid oxidation, lipid catabolism, and energy homeostasis. Provided at a working volume of 100 µl, the antibody is validated for western blot detection of endogenous PPARA across human, mouse, and rat samples.

The reagent supports cross-species studies of PPARA expression in oxidative tissues, including liver, heart, brown adipose tissue, and skeletal muscle. Typical research applications include investigations of non-alcoholic fatty liver disease, dyslipidemia, fasting metabolism, and pharmacological responses to fibrate treatment. Researchers commonly pair this reagent with qPCR or RNA-seq readouts to correlate protein levels with downstream targets such as CPT1A, ACOX1, and FABP1, clarifying the transcriptional regulatory activity of the receptor under different metabolic states.

For reproducible western blot signal, aliquot the antibody upon arrival and store at -20 °C, protected from light to maintain IgG integrity through repeated freeze-thaw cycles. The unconjugated format allows pairing with HRP- or fluorophore-labelled secondary antibodies for chemiluminescent or fluorescent detection of endogenous PPARA in whole-cell, nuclear, or tissue extracts. For research use only; not intended for diagnostic or therapeutic applications.