Anti-PPARA Antibody

The PPARA target (Peroxisome Proliferator-Activated Receptor Alpha) is a ligand-activated transcription factor belonging to the nuclear receptor superfamily. This unconjugated rabbit polyclonal IgG antibody from Antibodies.com is raised against PPARA and validated for Western blot detection across Human, Mouse, and Rat samples. Researchers commonly use it to study fatty acid oxidation, lipid metabolism, peroxisomal function, and energy homeostasis in liver, cardiac, and skeletal muscle models.

The antibody is supplied as 100 µl of polyclonal serum in liquid format; for long-term stability, aliquot and store at -20 °C, avoiding repeated freeze-thaw cycles. A recommended Western blot workflow includes loading 20-50 µg of total protein lysate per lane, separation by SDS-PAGE, transfer to nitrocellulose or PVDF membrane, and chemiluminescent readout. The unconjugated format allows pairing with secondary antibodies of choice (HRP, AP, or fluorophore-conjugated anti-rabbit IgG) for flexible detection schemes.

PPARA expression varies by tissue, with high levels in hepatocytes, cardiomyocytes, and renal tubular epithelium; differential expression under metabolic, inflammatory, or pharmacological stimuli makes this reagent useful for studies of PPAR agonists, fatty liver models, and metabolic disease research. Reactivity spans Human, Mouse, and Rat, supporting comparative work across species. This is a primary antibody intended for research use only, not for diagnostic or therapeutic applications.