Anti-PPARγ (phospho-S112) Antibody

This rabbit polyclonal antibody specifically recognizes PPARγ phosphorylated at serine 112 (phospho-S112), a regulatory modification that modulates the transcriptional activity of this nuclear receptor. Distributed by Antibodies.com, the reagent is raised in rabbit and validated for Western blot and immunohistochemistry, with confirmed reactivity against human, mouse, and rat samples. The unconjugated IgG is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, and is packaged as a 100 µl vial sized for short-term laboratory use. Researchers studying adipogenesis, insulin signaling, metabolic disease, or oncology frequently target the phospho-S112 epitope because ERK/MAPK-mediated phosphorylation at this residue dampens ligand-independent transactivation and influences adipocyte differentiation programs. The polyclonal format provides recognition of multiple epitopes on the phosphorylated target, which is advantageous when assessing modification state across varied lysate backgrounds. For storage, the antibody should be refrigerated; aliquoting is recommended to limit repeated freeze-thaw cycles that can degrade antibody integrity over time, and the 0.05% sodium azide acts as a preservative against microbial contamination.