Anti-PPA1 Antibody

This rabbit polyclonal antibody from Antibodies.com targets PPA1 (Pyrophosphatase (Inorganic) 1), a magnesium-dependent cytoplasmic enzyme that hydrolyzes inorganic pyrophosphate into two orthophosphate molecules. PPA1 supports cellular phosphate homeostasis and drives biosynthetic reactions that generate pyrophosphate as a byproduct. The unconjugated reagent is validated for western blot and immunohistochemistry applications across human, mouse, and rat samples, supplied as 100 µl of polyclonal antiserum raised in rabbit hosts.

PPA1 function has been studied in cartilage and bone biology, where its pyrophosphatase activity influences extracellular matrix mineralization and ectopic calcification. Altered PPA1 expression has also been reported in several cancer cell lines and primary tumor tissues, linking inorganic pyrophosphate hydrolysis to proliferative and metabolic pathways. The polyclonal format offers multi-epitope recognition of the target, useful when probing conserved regions across species or detecting isoforms that differ in post-translational modification.

For western blot, the reagent detects endogenous PPA1 in lysates prepared from cultured cells or fresh and frozen tissue. In immunohistochemistry, it can be applied to formalin-fixed paraffin-embedded sections to localize PPA1 at the cellular and subcellular level. The unconjugated format gives researchers flexibility to pair the reagent with their preferred secondary antibody, fluorophore, or enzyme conjugate.