Anti-POU5F1 Antibody

This Mouse monoclonal antibody targets POU5F1 (also known as Oct-4), a homeodomain transcription factor critical for maintaining pluripotency in human embryonic stem cells and induced pluripotent stem cells. From Antibodies.com, the unconjugated IgG1 reagent supports western blot, immunocytochemistry, and flow cytometry workflows used in stem cell biology and reprogramming research.

Validated for human samples, the reagent is supplied as 100 µl of antibody solution. Typical uses include tracking Oct-4 expression during reprogramming experiments, monitoring pluripotency marker status in hESC and iPSC cultures, and flow cytometric analysis of stem cell populations expressing the pluripotency transcription factor. The Mouse host and monoclonal clonality provide lot-to-lot consistency for quantitative comparisons across experiments.

Store refrigerated and aliquot to limit freeze-thaw cycles; for extended storage, freeze the antibody preparation to preserve binding activity over repeated experiments. Protect from light and handle on ice when preparing working dilutions for immunocytochemistry.