Anti-POU2F2 Antibody

Raised in rabbit against the POU class 2 homeobox 2 protein, this polyclonal primary antibody from Antibodies.com recognizes POU2F2 — also widely cited as Oct-2, OTF-2, or NF-A2 — a member of the POU-homeodomain family of transcription factors. The reagent is supplied as 50 µl of unconjugated rabbit IgG antiserum and is validated for Western blot and immunohistochemistry workflows on human, mouse, and rat samples.

POU2F2 is a roughly 51 kDa nuclear DNA-binding protein that regulates immunoglobulin and other immune-gene promoters through the octamer motif, with markedly elevated expression in germinal-center B cells and in germinal-center-derived B-cell lymphomas. Because the polyclonal format spreads reactivity across multiple epitopes within the POU2F2 protein, the reagent is especially helpful for probing isoforms and post-translationally modified forms that diverge near the immunogen region.

For handling, store the vial at -20 °C in single-use aliquots to limit freeze-thaw cycles, and protect from light if a fluorescent conjugate is later added. Pair the primary with an anti-rabbit IgG secondary matched to the desired reporter — HRP, biotin, or a fluorophore — to drive chemiluminescent, chromogenic, or fluorescence readout on Western blots of whole-cell lysates (Ramos, Raji, Daudi, and Jurkat are common reference lines for POU2F2) or on fixed tissue sections probed by immunohistochemistry. Researchers studying B-cell maturation, octamer-driven promoter activity, POU-family transcription factor cross-talk, and lymphoma biology find this reagent useful for both nuclear localization in tissue and band detection on immunoblots.