Anti-POMGNT2 Antibody

This polyclonal antibody recognizes POMGNT2, a Golgi-resident glycosyltransferase that catalyzes the addition of N-acetylglucosamine during O-mannosylation of alpha-dystroglycan. Supplied by Antibodies.com as 200 µl of unconjugated rabbit IgG, the reagent is intended for immunofluorescence (IF) workflows on human specimens and supports research into dystroglycanopathies, congenital and limb-girdle muscular dystrophy, Walker-Warburg syndrome, and broader protein glycosylation biology.

Because polyclonal preparations engage multiple epitopes on the target, this antibody is well matched to detection of the full-length POMGNT2 protein in fixed cells or cryosections, including samples where post-translational modification, splice variation, or low expression compromise monoclonal recognition. For routine handling, aliquot the concentrate into single-use vials and store at -20 °C; protect working dilutions from light and limit freeze-thaw cycles to preserve binding activity. Short-term refrigerated storage is acceptable for active bench aliquots.

Typical starting dilutions lie in the 1:100 to 1:500 range for IF on paraformaldehyde-fixed cells, with further optimization recommended against each specific sample preparation and detection system. The rabbit host enables clean pairing with mouse, goat, or chicken secondary antibodies in multiplex fluorescence imaging panels, and the unconjugated format leaves the researcher free to select fluorophore, biotin, or HRP conjugates as the experiment demands.