Anti-POLR3A Antibody

This unconjugated polyclonal antibody from Antibodies.com specifically recognizes POLR3A (RNA Polymerase III Subunit A), the catalytic largest subunit of human RNA polymerase III. Raised in rabbit and purified as an IgG preparation, it is supplied at 50 µl per vial and is validated for Western blot detection of the ~160 kDa endogenous subunit in lysates of human origin.

POLR3A drives transcription of small structural RNAs, including 5S rRNA, tRNAs, and U6 snRNA, making this reagent useful for studies of core transcription machinery, ribosome biogenesis, and the molecular basis of POLR3A-related leukodystrophies such as 4H syndrome and Wiedemann-Rautenstrauch syndrome. Researchers investigating transcriptional dysregulation, nucleolar function, or genetic disorders linked to POLR3A mutations can use this reagent to confirm expression and track subunit levels across cell lines and patient-derived samples.

Provided as a single-use aliquot of unconjugated IgG, the antibody offers flexibility for downstream labeling with secondary reporters in Western blot workflows. Store refrigerated, protect from light to preserve activity, and minimize freeze-thaw cycles to maintain binding performance.