Anti-POLR3A Antibody

Produced in rabbit hosts and supplied unconjugated for flexibility across detection platforms, this polyclonal reagent from Antibodies.com targets POLR3A, the catalytic subunit of RNA Polymerase III responsible for transcribing tRNAs, 5S rRNA, and other small structural RNAs in human cells. Validated for Western blot application, the IgG isotype preparation is supplied as 200 µl aliquots suitable for routine immunoblotting of endogenous POLR3A protein.

The immunogen corresponds to a region of the POLR3A polypeptide, and affinity-purification of the resulting antiserum yields a reagent that recognizes a band at the expected molecular weight in human cell lysates. Laboratories investigating transcription, ribosome biogenesis, and POLR3A-associated disease mechanisms use this antibody in studies ranging from basic RNA Polymerase III function to clinical correlations with conditions such as Treacher Collins syndrome and Wiedemann-Rautenstrauch syndrome, in which POLR3A mutations are implicated.

Aliquot the antibody upon receipt and store at -20°C in a frost-free freezer; avoid repeated freeze-thaw cycles to preserve binding activity. The unconjugated format permits pairing with HRP-conjugated, fluorescent, or biotinylated secondary antibodies according to experimental design. This Primary Antibody is intended for research use only in immunoblotting workflows against human-derived samples.