Anti-POLR2J Antibody

Raised in rabbit, this unconjugated polyclonal antiserum from Antibodies.com targets POLR2J, the ~13 kDa J subunit of human RNA polymerase II that integrates into the twelve-subunit Pol II complex responsible for mRNA synthesis. Researchers working on eukaryotic transcription use it to probe polymerase subunit composition, transcription-coupled processing, and CTD phosphorylation states. The reagent is supplied as 50 µl of antiserum in a single vial and has been validated across three independent readouts: Western blot for the POLR2J band, immunohistochemistry on fixed tissue sections, and immunofluorescence imaging in cultured cells. Confirmed reactivity covers human, mouse, and rat samples, so the same aliquot can support comparative work between human cell lines and rodent models. For best stability, aliquot on arrival and store at -20 °C, avoiding repeated freeze-thaw cycles. Each lot is affinity purified and quality controlled, with documentation covering host species, clonality, conjugate status, and target specificity. Intended for research use only.