Anti-POLR2I Antibody

This polyclonal reagent from Antibodies.com is raised in rabbit against POLR2I, the ninth subunit (RPB9) of RNA polymerase II. The subunit sits within the Pol II jaw-lobe and contributes to the active site geometry that positions the incoming template strand during initiation of mRNA and snRNA synthesis, making it a useful marker for studying the core transcription machinery at subunit resolution.

Validated for Western blot on human, mouse, and rat samples, the unconjugated antibody is supplied as 100 µL at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide, at approximately pH 7.2. The concentrated stock permits flexible dilution for standard immunoblotting of whole-cell and tissue lysates in transcription and chromatin research workflows.

Researchers examining Pol II assembly, elongation dynamics, or stress-induced changes in subunit stoichiometry can use this reagent alongside total Pol II markers to resolve RPB9-specific bands. Refrigerated storage preserves activity across multiple working sessions; aliquot on first use to limit freeze-thaw cycles and protect antibody integrity for downstream blotting experiments.