Anti-POLR2F Antibody

This Rabbit polyclonal IgG reagent from Antibodies.com targets POLR2F, the sixth subunit of mammalian RNA Polymerase II. Raised against the human protein, the unconjugated antibody is supplied as 200 µl of liquid and supports detection by Western blot and immunofluorescence workflows. POLR2F (also called RPB6) forms part of the RNA Pol II core and contributes to assembly and stability of the polymerase complex, making the reagent useful for studies of transcription machinery composition, Pol II subunit stoichiometry, and nuclear organization in human cell lines and primary cultures. The polyclonal format offers recognition of multiple epitopes on POLR2F, and the unconjugated IgG works with standard secondary antibody labeling, including HRP- and fluorophore-conjugated secondaries for chemiluminescent or fluorescent imaging. Researchers investigating transcription regulation, RNA Pol II assembly, or chromatin-associated complexes can apply the reagent to whole-cell or nuclear extracts in WB and to fixed cells in IF, with reactivity confirmed against the human antigen.