Anti-POLR2C Antibody

Generated in rabbit, this polyclonal reagent targets the third-largest subunit of human RNA polymerase II, the 33 kDa protein POLR2C (also called RPB3), which sits at the core of the complex that transcribes protein-coding genes. Antibodies.com ships the unconjugated antibody at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant, and the 100 µl vial is sized for routine western blot loading and immunohistochemistry on fixed sections. Validated reactivity covers human, mouse, and rat samples, so a single lot can support cross-species comparison of polymerase II expression in cultured cell lines, primary tissue, and animal model work. Because the antigen is conserved across these species, the antibody is widely used to study transcription-coupled processes including recruitment of polymerase II to promoters, response to DNA damage, and changes in expression during differentiation or cellular stress. Store the glycerol-containing liquid at -20 °C in working aliquots to limit freeze-thaw cycles; the sodium azide permits short-term handling at 4 °C without microbial contamination. This Primary Antibodies reagent is supplied as research use only and is well suited to labs running parallel human and rodent panels on a single blot.