Anti-POLR2A Antibody

Raised in rabbit and distributed by Antibodies.com, this unconjugated polyclonal reagent binds POLR2A, the largest catalytic subunit of RNA polymerase II that synthesizes messenger RNA and many small nuclear RNAs in eukaryotic nuclei. Provided at 100 µl per vial, it supports western blotting, immunohistochemistry on fixed tissue, and immunofluorescence imaging of cultured cells, with reactivity confirmed against human, mouse, and rat samples. Researchers use it to probe C-terminal domain phosphorylation states, track transcription elongation complexes, monitor recruitment to active chromatin, and compare polymerase II loading between treated and untreated conditions. The polyclonal format broadens epitope coverage along the Rpb1 backbone, an advantage when studying post-translational modifications distributed across heptad repeats of the CTD.