Anti-POLR1C Antibody

This rabbit polyclonal antibody from Antibodies.com targets POLR1C, the shared subunit of RNA Polymerase I and RNA Polymerase III. Raised in rabbit and supplied as an unconjugated immunoglobulin fraction, the reagent is validated for Western Blot detection of the endogenous protein in lysates prepared from human, mouse, and rat samples. Each vial contains 100 µl at a concentration of 1 mg/ml in PBS with 0.1% sodium azide as preservative and 50% glycerol as cryoprotectant, providing laboratories with a stable working stock for repeated immunoblotting experiments.

POLR1C functions within the nucleolus, where it contributes to ribosomal RNA synthesis by RNA Polymerase I and supports 5S rRNA and tRNA production through its role in RNA Polymerase III. Investigators studying ribosome biogenesis, nucleolar stress responses, and transcriptional regulation rely on robust antibodies to monitor POLR1C expression across developmental stages and disease models. Loss-of-function mutations in POLR1C are linked to Treacher Collins syndrome, a disorder of craniofacial development, as well as a form of hypomyelinating leukodystrophy called POLR1C-related leukodystrophy, making the protein a target of interest for groups investigating these rare genetic conditions.

For Western Blot, the antibody pairs with standard HRP-conjugated secondary reagents and either chemiluminescent or chromogenic substrates to reveal a band near the expected molecular weight of the POLR1C protein. Aliquoting upon first use and storing working volumes at -20 degrees C helps preserve activity across multiple freeze-thaw cycles. Cross-reactivity with human, mouse, and rat POLR1C offers flexibility for comparative studies across cell line panels, primary tissue extracts, and animal model systems used in basic and translational research.