Anti-POLK Antibody

POLK (DNA Polymerase Kappa) is a Y-family translesion synthesis polymerase best known for bypassing bulky DNA adducts such as BPDE-N2-dG lesions and supporting error-prone replication across damaged templates. This polyclonal rabbit IgG reagent from Antibodies.com is supplied unconjugated at 100 µl per vial and is listed for Western blot and immunofluorescence workflows on human, mouse, and rat samples.

Researchers use this reagent to investigate error-prone DNA synthesis, mutagenesis, the regulation of translesion synthesis by PCNA ubiquitination, and the role of POLK in cellular responses to DNA-damaging agents. On Western blot of whole-cell lysates, the polyclonal IgG detects the endogenous polymerase band, while on fixed cells immunofluorescence reveals its characteristic nuclear localization and recruitment to stalled replication foci. Polyclonal detection offers tolerance for minor sequence variation, useful when comparing POLK signal across species or in matched lysate and IF panels from the same experiment.

For the liquid format, short-term refrigerated storage protected from light is appropriate for active use; for longer-term storage, freeze in single-use aliquots and minimize freeze-thaw cycles by dividing the stock on first thaw. The unconjugated reagent leaves the choice of label open, allowing laboratories to conjugate to HRP, Alexa Fluor dyes, biotin, or gold nanoparticles as needed for downstream read-outs. Cross-reactivity outside the listed human, mouse, and rat profile has not been established, so applications in other species should be validated empirically before use in assays or staining protocols.