Anti-POLI Antibody

The Anti-POLI Antibody from Antibodies.com is a rabbit polyclonal reagent raised against DNA Polymerase Iota (POLI), a Y-family translesion synthesis polymerase that performs error-prone nucleotide incorporation across damaged DNA templates and contributes to mutagenesis and genome stability pathways.

This unconjugated primary antibody is validated for western blot (WB) and immunohistochemistry (IHC) workflows, enabling protein expression profiling in cell and tissue lysates as well as localization studies in fixed tissue sections. Reactivity is reported for human, mouse, and rat samples, supporting comparative work across established cell line models and rodent tissue specimens. The reagent is supplied as 100 µl of polyclonal antiserum and can be paired with standard HRP- or fluorophore-conjugated secondary antibodies for chromogenic, chemiluminescent, or fluorescent detection.

Researchers investigating DNA damage tolerance, translesion synthesis mechanisms, or the contribution of POLI to tumor biology will find this antibody useful for probing polymerase expression under genotoxic stress conditions and for mapping tissue distribution in normal versus diseased specimens.