Anti-POLE3 Antibody

Raised in rabbit against POLE3, the 17 kDa histone-fold accessory subunit of DNA polymerase epsilon, this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as a 100 µl volume and detects endogenous POLE3 protein in human and mouse samples by western blot and immunofluorescence.

POLE3, also called p17 and CHRAC-17, is a 147-amino-acid subunit that pairs with POLE4 inside the DNA polymerase epsilon holoenzyme and is independently incorporated into the CHRAC chromatin-remodeling complex and the ATAC histone acetyltransferase complex, where it supports DNA replication, nucleosome assembly, and chromatin accessibility. Because the unconjugated primary leaves label choice open, laboratories can pair it with their own HRP, Alexa Fluor, or biotin-conjugated secondary antibody depending on whether the downstream read-out is chemiluminescent western blot or fluorescent imaging of nuclear POLE3 distribution.

The polyclonal format increases the chance of detection across regions of the 17 kDa target, which is useful when probing chromatin-enriched nuclear fractions, subcellular compartments, or fixed-cell preparations. For short-term use, refrigerate the vial in its glycerol-containing buffer; for longer projects, aliquot to limit freeze-thaw cycles. The reagent is intended for research use only and is not validated for diagnostic applications.