Anti-POLD3 Antibody

This rabbit polyclonal antibody from Antibodies.com recognizes POLD3, the p66 accessory subunit of human DNA polymerase delta. POLD3 stabilizes the holoenzyme on chromatin and supports lagging-strand DNA synthesis, while also engaging in mismatch repair and translesion synthesis, so antibodies against it are commonly used in DNA replication, DNA repair, and genome-stability research.

Supplied as an unconjugated liquid reagent in a 100 µL volume, the antibody is validated for Western blot (WB) and immunofluorescence (IF) on human samples. The polyclonal format offers recognition of multiple epitopes on the target, which can be useful when probing samples where post-translational modification or isoform expression may vary. Because the reagent is unconjugated, it can be paired with a wide choice of secondary antibodies or custom labels to fit individual assay designs.

Typical uses include confirming POLD3 expression levels by immunoblotting, tracking subcellular localization by IF in proliferating cells, and supporting studies of replication fork stability and DNA damage response pathways. Researchers working with tumor cell lines, primary cultures, or model systems where polymerase delta activity is altered can use this antibody as a tool for characterizing POLD3 across experimental workflows.