Anti-polA Antibody

DNA polymerase I encoded by the polA gene of E. coli performs 5' to 3' polymerization coupled with 3' to 5' proofreading and 5' to 3' nick translation, and this Antibodies.com reagent supplies a rabbit polyclonal antibody for its detection. Validated for western blot and immunohistochemistry, the unconjugated preparation arrives as 100 µg per vial of polyclonal sera raised against the polymerase target. Researchers investigating prokaryotic DNA replication, Okazaki fragment maturation, and repair pathways can apply the antibody in bacterial lysate immunoblots and fixed-section staining protocols. The unconjugated format allows pairing with HRP, alkaline phosphatase, or fluorophore-labeled secondaries matched to each experimental readout. Refrigerated handling and brief equilibration to room temperature before use help preserve binding activity across multiple blotting sessions.