Anti-POFUT1 Antibody

For western blot detection of POFUT1 in human- and mouse-derived samples, this rabbit polyclonal primary antibody combines IgG isotype specificity with broad recognition of multiple epitopes. POFUT1 is an endoplasmic-reticulum-associated O-fucosyltransferase that transfers fucose to serine or threonine residues on folded EGF-like repeats. Its activity is especially relevant to the maturation and signaling of Notch-family receptors.

The Antibodies.com antibody is unconjugated and supplied in a 50 µL pack, allowing researchers to select an appropriate labeled rabbit-specific secondary reagent and chemiluminescent, fluorescent, or chromogenic detection system. Practical applications include examining POFUT1 abundance in cell or tissue lysates, investigating Notch receptor glycosylation and maturation, and supporting studies of developmental signaling pathways.

Use suitable positive and negative expression controls and optimize lysate preparation, protein transfer, blocking, and antibody dilution for the selected detection chemistry. For research use only; follow the current lot-specific datasheet for recommended storage, handling, and working dilution.