Anti-PNPT1 Antibody

This polyclonal antibody from Antibodies.com targets PNPT1 (Polyribonucleotide Nucleotidyltransferase 1), a mitochondrial exoribonuclease that degrades RNA from the 3' end and contributes to mitochondrial RNA processing, surveillance, and the import of small RNAs into the mitochondrial matrix. Raised in rabbit, the unconjugated reagent is supplied at 100 µL and is validated for Western blot (WB) and immunohistochemistry (IHC) workflows. The antibody recognizes the human and mouse PNPT1 orthologs, supporting translational studies that compare mitochondrial RNA metabolism between rodent models and human tissue samples.

PNPT1 is a focus of mitochondrial disease research, where loss-of-function variants have been linked to combined oxidative phosphorylation deficiency and to neurological phenotypes such as Leigh syndrome and hereditary hearing loss. Investigators studying mt-RNA turnover, mitochondrial stress responses, or PNPase-dependent control of mitochondrial transcripts use this reagent to probe endogenous protein levels in cell lysates and to localize expression in fixed tissue sections. The polyclonal format presents an epitope pool broad enough to track the full-length mitochondrial isoform, while unconjugated chemistry leaves the antibody compatible with HRP-, fluorophore-, or biotin-based secondary detection systems selected by the end user.

For short-term handling, the liquid antibody should be kept cold and protected from light; long-term storage at -20 °C in working aliquots helps preserve activity across freeze-thaw cycles. Each vial is intended as a single-use research reagent for laboratory studies and is not designated for diagnostic or therapeutic use.