Anti-PNP Antibody

Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine salvage pathway, catalyzing the phosphorolytic cleavage of inosine and guanosine to generate free bases and ribose-1-phosphate. Research-grade polyclonal antibodies raised against this target are used to study nucleoside metabolism, lymphocyte maturation, and the cellular consequences of PNP deficiency, which manifests as a T-cell immunodeficiency in humans.

This unconjugated rabbit IgG reagent from Antibodies.com is supplied as a 200 µl liquid aliquot and is validated for Western blot (WB) and immunoprecipitation (IP). It recognizes PNP in both human and mouse samples, supporting cross-species studies in lymphoid cell lines, primary T cells, and tissue lysates. The polyclonal format provides recognition of multiple epitopes on the homotrimeric PNP enzyme, which can be advantageous when probing denatured targets in WB or pulling down native complexes from whole-cell extracts in IP workflows.

Typical protocols begin with cell lysis in a non-denaturing buffer for immunoprecipitation, or in SDS-containing buffer for western blot separation and transfer to membrane. Investigators working on purine metabolism disorders, inherited immune deficiency phenotypes, or PNP expression in tumor models often pair this antibody with parallel enzymatic activity assays to confirm functional correlations.