Anti-PNLIP Antibody

Pancreatic lipase (PNLIP) is a key digestive enzyme secreted by pancreatic acinar cells that hydrolyzes dietary triglycerides into monoglycerides and free fatty acids, and this unconjugated rabbit polyclonal antibody from Antibodies.com is raised against the human protein for detection in western blot and immunofluorescence workflows.

The reagent ships as a 100 µl vial and is validated for western blot analysis of pancreatic tissue lysates and immunofluorescence staining of frozen or formalin-fixed pancreatic sections. Researchers studying exocrine pancreatic function, acute pancreatitis biomarkers, cystic fibrosis-related pancreatic insufficiency, and dietary fat metabolism routinely use anti-PNLIP reagents to track enzyme expression in acinar cells and to monitor circulating lipase levels during pancreatic injury.

Because the antibody is polyclonal, the host rabbit recognizes multiple epitopes across the PNLIP primary sequence, which can improve signal in western blot when the target is partially degraded or when working with samples where post-translational modification might mask individual epitopes. For storage and handling, the supplier recommends aliquoting the antibody upon arrival and freezing it at -20°C in working volumes to avoid repeated freeze-thaw cycles; for short-term use the vial may be refrigerated at 4°C.

The unconjugated format makes the reagent flexible for downstream labeling: it can be paired with anti-rabbit secondary antibodies conjugated to HRP, Alexa Fluor dyes, or biotin depending on the chosen application. Researchers studying PNLIP gene regulation, pancreatic acinar cell differentiation, or the role of pancreatic lipase in metabolic disease will find this antibody a useful primary reagent.