Anti-PNKP Antibody

Manufactured by Antibodies.com, this affinity-purified polyclonal reagent targets polynucleotide kinase 3'-phosphatase (PNKP), a key enzyme involved in the DNA damage response and single-strand break repair pathway. The antibody is raised in Rabbit and is supplied as an unconjugated preparation at 1.0 mg/mL, with the 50 µl size offered here being one of two available pack volumes. Immunisation was performed using a recombinant human PNKP protein as the immunogen, and the resulting IgG fraction was purified by antigen-affinity chromatography to ensure specificity for endogenous PNKP. Validated applications include Western blot (WB), immunocytochemistry / immunofluorescence (ICC/IF), and immunohistochemistry (IHC), giving researchers a versatile tool for both biochemical and tissue-based studies. Cross-reactivity has been confirmed in Human, Mouse, and Rat samples, which broadens its utility across a range of common model systems and primary cell work. The unconjugated format allows end-users to choose their own secondary labelling strategy, whether that be HRP, fluorophore, or biotin conjugates depending on the assay. For optimal preservation of activity, the antibody should be stored according to the manufacturer's recommendations, typically at -20 °C in working aliquots to avoid repeated freeze-thaw cycles. Each lot is supplied by AFMS through the Antibodies.com catalogue and is intended for research use only. Detailed lot-specific validation data and recommended working dilutions for each application are available on the manufacturer's product page.