Anti-PMS1 Antibody

PMS1 is a key component of the DNA mismatch repair (MMR) pathway, forming the MutLα heterodimer with MLH1 to correct base-base mismatches and small insertion/deletion loops generated during DNA replication. This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against PMS1 and validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human and rat samples.

Supplied as 100 µl of antibody at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, the reagent is ready to dilute into standard blocking and incubation buffers. The polyclonal format provides broad epitope coverage across the PMS1 protein, useful for detecting full-length expression as well as proteolytic fragments that may appear in MMR studies.

Typical applications include probing PMS1 expression in cultured mammalian cell lysates by WB and localizing the protein in formalin-fixed, paraffin-embedded tissue sections by IHC. Because PMS1 partners with MLH1, the antibody is often paired with anti-MLH1 reagents in co-immunoprecipitation and double-labeling experiments to interrogate MutLα complex integrity in mismatch repair-deficient tumors and in vitro DNA repair assays.

Storage at -20 °C in small aliquots is recommended to limit freeze-thaw cycles; the 50% glycerol formulation remains liquid at -20 °C for convenient bench handling. Sodium azide is included as a preservative and should be removed by buffer exchange or a spin column before conjugating or use in live-cell systems. This antibody is intended for research use only.