Anti-PMS1 Antibody

Generated in rabbit, this polyclonal primary antibody targets the PMS1 protein, a component of the DNA mismatch repair (MMR) machinery that corrects base-pair mismatches introduced during replication. Supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated reagent is ready to dilute into standard western blot and immunohistochemistry workflows.

Validated against human, mouse, and rat samples, the reagent supports cross-species studies of genomic stability, microsatellite instability, and Lynch syndrome–related research. In western blot, it detects endogenous PMS1 in whole-cell lysates, yielding a band near the predicted molecular weight on SDS-PAGE. For immunohistochemistry, it reveals PMS1 distribution in fixed tissue sections, including paired tumor and matched-normal comparisons where MMR status is the underlying question.

The 50% glycerol buffer keeps the antibody liquid and stable at -20°C across multiple freeze-thaw cycles, while the included sodium azide preserves sterility for routine bench handling. Aliquot upon first use to protect working stocks from repeated freeze-thaw stress.

Researchers investigating DNA repair pathways, hereditary cancer syndromes, or the cellular response to replication stress frequently pair this reagent with antibodies against MLH1, MSH2, and MSH6 to assemble a complete mismatch repair panel. The polyclonal format recognises multiple epitopes, which is helpful when PMS1 expression is variable or low in the sample. Each preparation is quality controlled by Antibodies.com and is delivered as a single 100 µl vial of liquid stock.