Anti-PMPCA Antibody

Mitochondrial protein import depends on the mitochondrial processing peptidase (MPP), a heterodimeric metalloprotease that removes N-terminal mitochondrial targeting presequences from precursor proteins once they reach the matrix. The alpha subunit of MPP is encoded by PMPCA, a matrix-localized metallopeptidase expressed across tissues. This rabbit polyclonal IgG antibody from Antibodies.com was raised against PMPCA and validated for Western blot detection of the endogenous protein in human, mouse, and rat samples.

Supplied as 100 µl of unconjugated antiserum, the reagent is suited to whole-cell lysates, tissue homogenates, and enriched mitochondrial fractions. Researchers working on mitochondrial biogenesis, oxidative phosphorylation, metabolic disease, or neurodegeneration linked to MPP dysfunction use it to monitor steady-state PMPCA expression and to track presequence cleavage intermediates generated during precursor import. The polyclonal format offers broad epitope coverage and consistent detection across species in comparative studies.

For short-term handling, refrigerate the vial at 4 °C; for longer storage, aliquot into single-use portions and keep frozen at -20 °C to limit freeze-thaw loss of activity. Refer to the lot-specific datasheet provided by Antibodies.com for recommended dilution, blocking conditions, and compatible lysate preparation.