Anti-PML Protein Antibody [B3E9]

PML nuclear bodies sit at the centre of multiple cell biology research areas, and the B3E9 monoclonal antibody from Antibodies.com provides a defined reagent for tracking the Promyelocytic Leukemia scaffold protein in human cells. Supplied as 100 µg of lyophilised powder in a single vial, this unconjugated mouse IgG1 antibody is suited to western blot detection of endogenous PML isoforms prepared from whole-cell lysates. The clone binds selectively to human PML, enabling researchers to follow SUMO-modified and unmodified forms of the protein that drive nuclear body assembly, the DNA damage response, and downstream regulation of p53 activity.

PML is a tumour suppressor best characterised by its involvement in acute promyelocytic leukemia, where the t(15;17) chromosomal translocation generates the PML-RARA fusion oncoprotein. Reagents such as this B3E9 clone support visualisation of the disrupted microspeckled nuclear body pattern that defines APL blasts, and they appear regularly in studies of viral restriction, type I interferon signalling, and arsenic trioxide-driven PML degradation. The lyophilised format allows refrigerated storage and stable reconstitution in PBS for immunoblotting workflows, with detection typically achieved by chemiluminescent readout of bands resolved on SDS-PAGE gels.