Anti-PML Antibody

This polyclonal antibody, supplied by Antibodies.com as an unconjugated rabbit host reagent, specifically recognizes the human Promyelocytic Leukemia (PML) protein. PML organizes PML nuclear bodies, subnuclear compartments involved in DNA damage response, intrinsic antiviral defense, transcriptional regulation, and p53-mediated tumor suppression. Validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows, the reagent allows researchers to monitor PML expression, subcellular localization, and nuclear body integrity across diverse experimental readouts.

Each vial contains 100 µL of liquid-format antisera, supplied unconjugated to permit flexible pairing with user-selected secondary labels or fluorophores. Raised in rabbit and tolerant of standard immunolabeling conditions, the antibody fits both fixed-cell imaging of PML body assembly and biochemical detection of endogenous PML from human cell lysates. Investigators studying acute promyelocytic leukemia biology, where PML function is disrupted by the t(15;17) translocation that generates the PML-RARA fusion oncoprotein, rely on this reagent to track nuclear body reorganization during differentiation therapy and arsenic trioxide treatment studies.

Best practice is to aliquot the antibody upon receipt to limit freeze-thaw cycles and preserve performance across multiple experiments. The reactivity profile is restricted to human PML, supporting focused studies of patient-derived samples and engineered human cell lines in which PML signaling is the variable under investigation.