Anti-PLOD3 Antibody

Distributed as a 200 µl vial of unconjugated IgG produced in rabbit, this polyclonal Primary Antibodies reagent from Antibodies.com targets PLOD3 and is validated for Western blot work on human and mouse samples. PLOD3 (procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3) is a member of the lysyl hydroxylase family that localizes to the endoplasmic reticulum and catalyzes hydroxylation of lysine residues in collagen-like peptides. That post-translational modification is required for stable intermolecular cross-linking of fibrillar collagens and proper basement-membrane assembly, making the enzyme a focal point in connective-tissue, extracellular-matrix, and cancer-biology research.

The unconjugated format allows laboratories to pair the reagent with their own secondary detection system, while the polyclonal IgG composition provides multi-epitope recognition that is useful when probing endogenous PLOD3 levels in whole-cell lysates and tissue homogenates run under reducing SDS-PAGE conditions. Host species is rabbit and reactivity spans both human and murine specimens, supporting comparative studies between clinical material and mouse models of disease.

For routine handling, the antibody is supplied in a 200 µl vial and should be aliquoted on first use to limit freeze-thaw cycles, with diluted working solutions stored refrigerated. The reagent is configured as a ready-to-use immunoblotting product sized for typical Western blot workloads, supporting multiple membrane-probing experiments from a single tube of stock.