Anti-PLK1 (Phospho-Ser137) Antibody

From Antibodies.com, this rabbit polyclonal antibody is raised against PLK1 (Phospho-Ser137) and is validated for Western blot (WB). It is unconjugated and reacts with human, mouse, and rat samples.

The antibody was produced by immunizing rabbits with a synthetic phosphopeptide corresponding to the sequence around the phosphorylation site of serine 137 (R-R-S(p)-L-L) derived from human PLK1, conjugated to KLH. Purification was carried out by affinity chromatography using the epitope-specific phosphopeptide, and non-phospho-specific antibodies were removed by chromatography using the non-phosphopeptide. The reagent specifically detects endogenous levels of PLK1 only when phosphorylated at serine 137.

Supplied as a liquid at 1.0 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. The antibody is shipped at 4°C; upon delivery it should be aliquoted and stored at −20°C, and freeze/thaw cycles should be avoided.