Anti-PLK1 Antibody

This polyclonal reagent from Antibodies.com targets PLK1 (Polo-like kinase 1), a serine/threonine kinase that governs mitotic entry, spindle assembly, and cytokinesis. Researchers studying cell division, checkpoint control, and oncogenic signaling rely on reliable PLK1 detection to profile proliferating populations and tumor models.

Supplied as 100 µl of unconjugated rabbit antiserum, the antibody binds native and recombinant PLK1 and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows. The unconjugated format gives labs freedom to conjugate the reagent to HRP, biotin, or fluorophores of their choice, matching established in-house protocols.

Reactivity covers Human, Mouse, and Rat samples, supporting translational studies that span patient-derived tissue, rodent disease models, and standard cell line panels. Polyclonal recognition broadens epitope coverage, which is helpful when probing for truncated or post-translationally modified PLK1 isoforms.

Typical uses include tracking PLK1 levels across the cell cycle in HeLa or U2OS synchronization experiments, mapping kinase localization at centrosomes and kinetochores by IF, and confirming knockdown or inhibitor treatment effects in xenograft lysates by WB. For IHC, the reagent performs well on formalin-fixed paraffin-embedded sections of proliferative and neoplastic tissue.

Storage and handling: aliquot upon arrival to limit freeze-thaw cycles and store refrigerated; protect from light if downstream conjugations are planned. This product is intended for research use only and is not validated for diagnostic or therapeutic applications.