Anti-PLD2 (phospho-Y169) Antibody

Phospholipase D2 (PLD2) sits at the heart of phosphatidic acid signaling, vesicle trafficking, and cytoskeletal remodeling, and phosphorylation at tyrosine 169 is a regulatory event that controls its activity downstream of receptor and Src-family kinases. This rabbit polyclonal antibody from Antibodies.com was raised against a phosphorylated peptide encompassing the Y169 site, providing selective recognition of the activated, tyrosine-phosphorylated form of PLD2 rather than total protein.

The reagent is supplied as 100 µl at 1 mg/ml in phosphate-buffered saline (pH 7.2) containing 0.05% sodium azide as a preservative. It is unconjugated, leaving the choice of secondary reporter and labeling chemistry open for Western Blot detection. Validated reactivity spans human, mouse, and rat samples, so lysates from cell lines, primary cultures, and tissues can be analyzed side by side in comparative signaling studies.

Common uses include monitoring phospho-PLD2 induction in response to growth factor or integrin stimulation, mapping PLD2 activation in cancer cell migration and invasion assays, and characterizing the contribution of PLD2-mediated lipid signaling to membrane trafficking and cytoskeletal dynamics. For storage, working aliquots should be refrigerated while stocks are best kept frozen in single-use portions to limit freeze-thaw cycles.