Anti-PLCg2 (Ab-753) Antibody

PLCg2 (phospholipase C gamma 2) is a phospholipase family signaling enzyme best known for its role in B-cell receptor signaling, NK cell receptor activation, and mast cell degranulation, where it hydrolyzes PIP2 to generate the second messengers IP3 and DAG. This rabbit polyclonal antibody from Antibodies.com is raised against a peptide epitope in the region surrounding residue 753 of human PLCg2.

Validated for Western blot (WB) and immunofluorescence (IF), the reagent detects endogenous PLCg2 in human, mouse, and rat samples. The unconjugated polyclonal antiserum is supplied as 100 µl, ready for direct use in standard immunodetection workflows or for downstream custom conjugation. The polyclonal format enables recognition of multiple epitopes on the target protein, offering broader tolerance for minor species variation than a typical monoclonal reagent.

Typical research uses include profiling PLCg2 expression and phosphorylation in lymphoid tissue lysates and B-cell or NK cell lines, and studies of immune dysregulation disorders such as PLAID and APLAID, in which germline PLCG2 variants alter enzyme regulation and downstream calcium mobilization. Researchers also deploy the antibody to confirm CRISPR-mediated knock-out or knock-down phenotypes affecting PLCg2 levels by Western blot across primary cells and cultured lines.